NEBNext FFPE DNA Repair Mix – Repair and Prepare DNA from FFPE Samples
The NEBNext FFPE DNA Repair Mix is specifically designed to repair damaged DNA extracted from formalin-fixed, paraffin-embedded (FFPE) tissue samples. This specialized mix restores nicks, abasic sites, and other DNA lesions, producing high-quality DNA suitable for downstream molecular biology applications such as library preparation for next-generation sequencing (NGS), PCR, and qPCR.
By efficiently correcting common FFPE-associated DNA damage, this kit enhances the reliability and reproducibility of genomic analyses, making it essential for clinical research, oncology studies, and archival sample investigations.
✅ Key Features
- Repairs fragmented and damaged DNA from FFPE samples
- Restores nicks, abasic sites, and oxidative lesions
- Produces high-quality DNA suitable for PCR and NGS library prep
- Compatible with low-input and precious samples
- Optimized for clinical research and archival tissue studies
📦 Kit Contents
- DNA Repair Mix enzymes
- Optimized reaction buffer
- Protocol for FFPE DNA repair
🔬 Applications
- NGS library preparation from FFPE samples
- PCR and qPCR analysis of damaged DNA
- Clinical research and oncology studies
- Archival tissue DNA analysis
- Genomic and functional genomics research
❓ FAQ
1. What type of DNA damage does this kit repair?
👉 It repairs nicks, abasic sites, oxidative damage, and other common lesions in FFPE-extracted DNA.
2. Is it suitable for low-input DNA samples?
👉 Yes, the kit works efficiently with low DNA concentrations, making it ideal for precious or limited samples.
3. Can repaired DNA be used for NGS library preparation?
👉 Absolutely. Repaired DNA is fully compatible with downstream NGS workflows and PCR amplification.